Ts65Dn Information
Cesium irradiation was used to produce a reciprocal translocation, T65Dn, in which the distal end of mouse Chr 16 is carried in a small marker chromosome. T/+ females produced progeny trisomic for this small translocation product and a stock was established in which Ts65Dn mice are trisomic for the genes carried in the small translocation chromosome, symbolized Ts(1716)65Dn. T65Dn translocation breaks mouse Chr 16 just proximal to the amyloid precursor protein (App) gene and contains the HSA21-homologous genes from App to the telomere. The Ts65Dn chromosome also contains the centromere and a small portion (~5%) of Chr 17. Northern and Western blotting and enzyme activity assays demonstrate that genes on the translocation product are expressed at elevated levels in segmentally trisomic animals. Fluorescent in situ hybridization (FISH) to chromosomes and mapping in somatic cell hybrids have been used to define the chromosomal content of the trisomic segment.
| Colony Maintenance | Somatic Cell Hybrid Mapping |
| Controls | Human / Mouse Homology Map |
| Photograph of Mice | Photograph of Chromosomes |
| FISH Experiments |
This Resource is supported by NICHD/NIH contract #N01-HD-73265.
