Segmentally trisomic Ts65Dn mice provide a postnatal model for Down syndrome. Cesium irradiation was used to produce a reciprocal translocation, T (16;17) 65Dn. One of the translocation chromosomes is a small marker chromosome, comprised of the centromere and proximal end of Chr 17 (~9.5Mb) and the distal end of mouse Chr 16 (~34Mb). Translocation heterozygous (T/+) females produced progeny trisomic for this small translocation product and a stock was established in which Ts65Dn mice are trisomic for the genes carried in the small translocation chromosome, symbolized Ts(1716)65Dn.
The precise locations of the Chr 16 and Chr 17 breakpoints are 84,351,351 bp and 9,426,822 bp, respectively. The Chr 16 segment contains about two thirds of the human Chr 21 homologues in the mouse, from mitochondrial ribosomal protein L39 (Mrpl39) gene to the distal telomere. These data were used to generate a PCR genotyping assay for Ts65Dn (Reinholdt et al., 2011), replacing the previous methods of chromosome analysis or qPCR. Northern and Western blotting, enzyme activity assays and reverse phase protein arrays (RPPA) demonstrate that some but not all genes in the translocation product are expressed at elevated levels in segmentally trisomic animals. RPPA shows a loss of correlation among some brain proteins (Ahmed et al., 2012).
Stock No. 001924 is the original trisomic Ts(1716)65Dn strain. However, Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this stock; Pde6brd1 homozygotes are blind. Stock No. 005252 is an alternative strain, with a virtually identical genetic background except that it is wild-type for Pde6b, the result of continuous backcrossing to (C57BL/6JEiJ x C3Sn.BLiA-Pde6b+/DnJ)F1/J.
Details on TS65Dn
|Colony Maintenance||Photograph of Chromosomes|
|FISH Experiments||Photograph of Mice|
|Human / Mouse Homology Map|
Reinholdt LG, Ding Y, Gilbert GT, Czechanski A, Solzak JP, Roper RJ, Johnson MT, Donahue LR, Lutz C, Davisson MT. 2011. Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn. Mamm Genome 22(11-12):685-91.
Ahmed MM, Sturgeon X, Ellison M, Davisson MT, Gardiner KJ. 2012. Loss of Correlations among Proteins in Brains of the Ts65Dn Mouse Model of Down Syndrome. J Proteome Res 11(2):1251-63.
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