Cytogenetic Protocol Supplies
|
Item |
Supplier |
Cat. No. |
Storage Temp |
Comments |
| RPMI medium with L-glutamine and hepes buffer | Invitrogen | 22400-097 (100ml) | 40C | Supplement with 1.0ml gentamycin |
| Purified Phytohaemagglutin (PHA) | Fisher |
R30852801(2 mg) |
Lyophilized 4C In Solution, -20C | For final concentration of 7.5ug/ml in a 1.5 ml culture use the following calculations: Dissolve 2mg PHA in 17.8ml of medium supplemented with 1.0ml gentamycin. Freeze in aliquots of 3-4ml. PI must have vital information on file and so state with each order. Note expiration date and request long shelf life when ordering. |
| Lipopolysaccharide (LPS) From E. Coli serotype 0111:B4 | Sigma | L2630 (25mg) | Lyophilized 4C In Solution, -20C | Dissolve 25mg LPS in 33.3ml of medium supplemented with 1.0ml gentamicin. Final concentration: 0.1ml = 50ug/ml in 1.5ml culture Freeze in aliquots of 3-4 ml. |
| Fetal Bovine Serum | Sigma | F2442 (100ml) | -20C | Arrives frozen - thaw - refreeze in aliquots of 3-4ml |
| Gentamicin 10mg/ml | Sigma | G1272 (10ml) | 4C | Expiration date on bottle |
| Sodum Heparin (10,000 USP units/ml) | Webster Inc. | 07-805-8262 (4ml vial) | 4C | Working solution: 0.5ml sodium heparin / 9.5ml medium supplemented with 1.0ml gentamicin. Make fresh after 1 week. |
| Colchicine | Sigma | C3915 (500mg) | 15 to 30C In Solution 4C | Stock solution: 0.5g colchicine / 100ml sterile distilled water Working solution: 0.1ml of 0.5% colchicine in 9.9ml saline = 50ug/ml |
| Tubes and Slides | |||
| Tubes with snap caps (Fisher brand) | Fisher | 14-956-3D | For blood collection and for freezing LPS, PHA and FBS |
| Corning 25760-16 | Fisher (16 x 125mm) (screw cap) | 14-959-14 | Polystyrene (untreated) 500/case |
| Kimax Brand Centrifuge Tubes | Fisher | K451505 | Put into these tubes to harvest |
| Fisher - Color Frost disp. slides | Fisher | 12550-43 yellow |
| Suppliers Address | Phone | Fax |
| Fisher Scientific, 52 Faden Road, Springfield, NJ 07081 | 800 766 7000 | |
| Remel, Inc., 12076 Santa Fe Dr., Lenexa, KS 66215-3594 | 800 255 6730 | |
| Sigma Chemical Company, P.O. Box 14508, St. Louis, MO 63178-9974 | 800 325 8070 | |
| Invitrogen | 800 828 6686 | |
| J A Webster Inc, 86 Leominster Road, Sterling, MA 01564 -2114 | Tel: 800 225 7911 | Fax: 978 422 0227 |
