FISH Experiments

Composite showing G-banding patterns

Composite showing G-banding patterns of the two translocation chromosomes in T65Dn/+ versus the FISH signals obtained with the Chr 16 and 17 paints. The probable origin of the various chromosomal segments is indicated.

Partial metaphase spread from a female T65Dn/+ mouse

Partial metaphase spread from a female T65Dn/+ mouse hybridized with the P1 clone #8940 for D17Mit19 on proximal Chr 17.

a) Note the fluorescent signal on the small 1716 translocation marker but not on the large 1617 translocation product.
b) Reverse image showing pseudo G-bands for chromosome identification in Fig. 2a. In Fig 2a and Figures 2c - f, chromosomes are counterstained with DAPI/actinomycin D and pseudocolored red.
c - f) Metaphase chromosomes from Ts65Dn mice following FISH with four different gene probes.
c) BAC #460-B-12 for Ncam2. Hybridization to Chr 16 is observed but not to the 1716 translocation chromosome
d) BAC #313-N-5 for Gabpa. Hybridization to both Chr 16 and the 1716 translocation chromosome is observed.
e) P1 clone #15-12 for App. Hybridization to both Chr 16 and the 1716 translocation chromosome is observed.
f) BAC #411-N-7 for Znf295. Hybridization to both Chr 16 and the 1716 translocation chromosome is observed.

Reference: Akeson EC, Lambert JP, Narayanswami S, Gardiner K, Bechtel LJ, Davisson MT. 2001. Ts65Dn – localization of the translocation breakpoint and trisomic gene content in a mouse model for Down syndrome. Cytogenet Cell Genet 93(3-4):270-6.


This Resource is supported by NICHD/NIH contract #HHSN275201000006.